PROOF trial

Penumbral Rescue by Normobaric O=O Administration in Patients With Ischaemic Stroke and Target Mismatch ProFile (PROOF)

The PROOF study, funded by the EU, is investigating whether high-dose oxygen therapy reduces the effects of stroke.

An ischaemic stroke is the most common type of stroke. The arteries that supply the brain with blood become clogged by clots. This kills the cells at the centre of the stroke and puts those nearby at great risk.



The longer the brain suffers from oxygen and blood deficiency, the more serious the consequences, and the more brain tissue will die. Emergency treatment must therefore get rid of the blocked arteries as quickly as possible.

Started in January 2017 at the Tübingen University Clinic and at eleven other clinical centres in eight European countries, the PROOF trial will test whether the consequences of stroke can be reduced by rapidly applying high-dose oxygen therapy to new patients.

The high-dose oxygen therapy is very simple and low-cost. The patient, after having been diagnosed with an ischemic stroke after their scan, wears a mask through which they inhale almost pure oxygen at a flow of 40 litres per minute. This keeps the oxygen content in the blood as high as it can be.

The aim is to ensure that the surrounding brain tissue at risk, which will have less oxygen but is not yet dead, is stabilised by the high oxygen content coming in. This continues until the clot is removed and the blood circulation is improved again. The treatment therefore aims at the so-called ‘shadow zones of the stroke, sometimes called the ‘rescue zones. These may include the entire stroke area in some patients.

PROOF is the first trial to thoroughly investigate high-dose oxygenation in stroke patients. If it proves that the brain can be saved by this, it could also bring great benefits to patients who are not near a clinic where stroke can be treated and who have longer access times to emergency care.

The study is coordinated by Dr Sven Poli, Senior Physician of the Department of Neurology with focus on neurovascular diseases at the University Hospital Tübingen. The European-wide study is funded by the European Union.

For more on the trial click here:

PROOF has received funding from the European’s Horizon 2020 research and innovation programme under grant agreement No 733379

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